Journal: Frontiers in Endocrinology

Article Title: Diagnostic Role of Prostate-Specific Membrane Antigen in Adrenocortical Carcinoma

doi: 10.3389/fendo.2019.00226

Figure Lengend Snippet: The staining of PSMA and CD34 in ACA and ACC (X200). As we can see from the figure, the staining of PSMA in CPA, APA, NFT and metastases were negative, while in ACC it was positive with high percentage.

Article Snippet: The slides were stained for CD34 (1:200; ZM-0046; Origene) and PSMA (diluted 1:100; ab19071; Abcam).

Techniques: Staining

Journal: Journal of Nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 10 7 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Molecular Therapy Nucleic Acids

Article Title: Ligand-modified rAAV6 vectors with nanoblades allow high level gene knock-in in HSPCs without compromising cell survival

doi: 10.1016/j.omtn.2025.102495

Figure Lengend Snippet: Figure 5. Coupling of rAAV6 with low amounts of mannose allowed high-level nanoblade-mediated knockin CD34+ cells without cell death induction (A) 8E4, 3E5, or 8E5 mannose sugars were incubated for 4 h at RT with 10E12 vg of rAAV6 vectors encoding for the donor template. 10E9 vg rAAV6 vector per condition were analyzed in dot blot using the ADK1 antibody for rAAV6 that recognizes the intact assembled capsid or conca- navalin A, which detected mannose bound to the capsid. (B) 5E8 vg of rAAV6 conjugated with indicated quantities of mannose were separated on a gel, followed by silver staining to reveal the intact capsids. (C and D) CD34+

Article Snippet: Purity of the selected CD34+ cell fraction was evaluated by fluorescence-activated cell sorting (FACS) analysis (FACSCanto, BD) with APC-conjugated anti-CD34 antibody (Miltenyi Biotech).

Techniques: Knock-In, Incubation, Plasmid Preparation, Dot Blot, Silver Staining

Journal: Diabetes, Metabolic Syndrome and Obesity

Article Title: Teneligliptin, a DPP-4 Inhibitor, Improves Vascular Endothelial Function via Divergent Actions Including Changes in Circulating Endothelial Progenitor Cells

doi: 10.2147/dmso.s403125

Figure Lengend Snippet: Figure 2 Mean endothelial progenitor cells (EPCs) between baseline and 28 weeks in the teneligliptin and control groups. Temporal changes in EPC levels from baseline to 28 weeks were not significantly different between the groups (P = 0.13). The bar graph and error bars show the mean and standard deviation, respectively.

Article Snippet: Briefly, EPCs were counted by measuring CD34 antibody-positive cells using a single-color flow cytometry assay, as previously described.14 Plasma levels of SDF-1α were measured using the Quantikine Human CXCL12/SDF-1α Immunoassay (R&D Systems, Minneapolis, MN, USA) with a commercially available enzyme-linked immunosorbent assay kit.

Techniques: Control, Standard Deviation